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- Why rt pcr test is done - why rt pcr test is done
RT-PCR test reverse-transcription polymerase chain reaction test is an established test for detecting viral infections as well as some tumours. In fact, doctors started using this test to diagnose COVID infection as early as January - just weeks after the world found out about this new coronavirus infection. Read on to know more about this test:. RT-PCR or reverse transcriptase-polymerase chain reaction is a diagnostic test that is used to look for the presence of nucleic acids, specifically RNA ribonucleic acid , in a given sample.
Nucleic acids are long-chain polymers of sugar, phosphate and a nitrogenous base. In a human body, DNA is responsible for coding all the traits - ranging from your eye colour to the colour of your hair and the number of toes you have. RNA, on the other hand, is a messenger, that carries the message code of the DNA so the cellular machinery can translate it and make proteins.
In a human cell, DNA stays in its place inside the nucleus - a small organ inside the cell. However, some viruses do not have DNA. Instead, their RNA serves as their genetic material. It is occasionally used in clinical settings to look for viral RNA.
RT-PCR is a much more effective method than looking directly for the presence of viral antigen specific proteins released by the virus against which the body produces an immune response since even a small amount of RNA can be used to diagnose the condition. Your doctor will tell you if you need to fast before the test.
Tell your doctor if you are taking any medicines, herbs or supplements as these may affect the results of this test. A nasopharyngeal nose or oropharyngeal mouth swab is taken to collect samples for COVID diagnosis. A swab is a long flexible rod usually made of plastic though it may also be a steel rod and having a tip made of rayon, polyester or flocked rayon. If you are getting tested for COVID , the technician will likely to take a nasopharyngeal or oropharyngeal swab sample.
Or the gene expression is normal - in case the test is done to look for a genetic condition. RT-PCR also indicates the viral load in the body - the amount of viruses present in the body.
This is very important since viral load is used to differentiate between active and persistent chronic infection in the body. The above information is provided from a purely educational point of view and is in no way a substitute for medical advice by a qualified doctor. En - English. Yoga And Fitness.
Other Topics. There may be a slight delay in the audio. Updated on April 23, RT-PCR test reverse-transcription polymerase chain reaction test is an established test for detecting viral infections as well as some tumours. To look for the presence of certain types of cancer and tumour RNA circulating in your bloodstream.
To detect the presence of certain genetic diseases. The blood sample for this test will be obtained from a vein in your arm. A nasopharyngeal swab is taken in the following way: You will first be asked to blow your nose into a tissue.
This will remove all the extra mucus from your nose and help them take the swab easily. Next, your doctor or a lab technician will ask you to tilt your head back to 70 degrees. The swab will be allowed to stay in this position for a few seconds so it can absorb any and all secretions. The swab will be put in a sterile vial and sent to a lab for analysis. For an oropharyngeal swab, the swabs are collected from the back of your mouth in a similar way. Depending on the results of this test, your doctor will determine the treatment.
Find Infectiologist in cities Infectiologist in Kheda. Consult a Doctor. Rio DC. Reverse transcription-polymerase chain reaction. Cold Spring Harb Protoc. Published Nov 3. PMID: Centers for Disease Control and Prevention [internet]. Ann Surg. J Clin Microbiol. Molecular Biology of the Cell. New York: Garland Science; Marty Francisco M. How to Obtain a Nasopharyngeal Swab Specimen. The New England Journal of Medicine.
Related Articles. Cholinesterase Test. Angiotensin-converting enzym Anti ds-DNA antibody Test. Antimitochondrial antibody Beta-2 microglobulin Serum Test. Read on app. Ask your health query now and get connected with a doctor within 10 minutes!
Why rt pcr test is done - why rt pcr test is done. RT-PCR tests: What it is and how it is done?
Also, negative results do not preclude the infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information. Only trained clinical laboratory personnel specifically instructed and trained in the techniques of real-time PCR and in vitro diagnostic procedures could carry out the RT-PCR Tests.
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They do not represent the views or opinions of newindianexpress. MotoGP rider thinks race was over, misses out on podium. Railways cancelled almost 9, train services in , over 1, due to coal movement: RTI. Home States Kerala. By Express News Service. Now we are on Telegram too. To look for the presence of certain types of cancer and tumour RNA circulating in your bloodstream. To detect the presence of certain genetic diseases. The blood sample for this test will be obtained from a vein in your arm.
A nasopharyngeal swab is taken in the following way: You will first be asked to blow your nose into a tissue. This will remove all the extra mucus from your nose and help them take the swab easily. Next, your doctor or a lab technician will ask you to tilt your head back to 70 degrees.
The swab will be allowed to stay in this position for a few seconds so it can absorb any and all secretions. The swab will be put in a sterile vial and sent to a lab for analysis. For an oropharyngeal swab, the swabs are collected from the back of your mouth in a similar way. Depending on the results of this test, your doctor will determine the treatment. Find Infectiologist in cities Infectiologist in Kheda.
Consult a Doctor. Rio DC. Reverse transcription-polymerase chain reaction. Cold Spring Harb Protoc. Published Nov 3. PMID: Centers for Disease Control and Prevention [internet]. Ann Surg. J Clin Microbiol. Molecular Biology of the Cell. New York: Garland Science; Marty Francisco M. How to Obtain a Nasopharyngeal Swab Specimen. The New England Journal of Medicine. Related Articles. Cholinesterase Test. Angiotensin-converting enzym Anti ds-DNA antibody Test.
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